protein a g magnetic beads  (MedChemExpress)

Article Title: PREX1 regulates renal fibrosis through the RPTOR-mTORC1 signaling pathway.
Article Snippet: For Co-IP assays, HK-2 cells were lysed in NP-40 buffer (Beyotime) containing protease and phosphatase inhibitors. .. Cell lysates containing 1 mg of total protein were incubated overnight at 4 ◦C with 5 μg of antiPREX1 antibody (Proteintech, Cat# 22460–1-AP) or control IgG (Proteintech, Cat# 30000–0-AP), followed by incubation with Protein A/G magnetic beads (MedChemExpress, Cat# HY-K0205) for 2 h at 4 ◦C. ..

Article Title: In vivo CRISPR screens identify dual functions of DNMT3A in mediating tumor immune evasion.
Article Snippet: Briefly, cells were lysed in RIPA buffer (Beyotime, P0013C) supplemented with phenylmethylsulfonyl fluoride (PMSF; MCE, HY-B0496) and phosphatase inhibitors I and II (MCE, HY-K0021, and HY-K0022). .. For IP assays, cell lysates were incubated with protein A/G magnetic beads (MCE, HY-K0202), anti-Flag magnetic beads (MCE, HY-K0207) or anti-DNMT3A antibody (ABclonal, A19659) overnight at 4 °C with gentle rotation. ..

Article Title: Targeting NSUN5-mediated m5C-RNA modification overcomes sunitinib resistance in renal cell carcinoma by suppressing Fibronectin/PI3K/AKT signaling
Article Snippet: In brief, mRNA was extracted with the Hieff NGS mRNA Isolation Master Kit (#12603ES24; Yeasen, China) and fragmented with 10× Fragmentation Reagent (AM8740; Ambion, USA) at 70 °C for 7 min. .. Following this, 5 μg of the anti-m5C antibody (#ab73938; Abcam) was combined with 50 μL of protein A/G magnetic beads (#HY-K0202; MCE) at RT for 30 min, followed by incubation with fragmented RNA at 4 °C overnight. ..

Article Title: KDM4B-mediated AP2A1-K217 demethylation promotes immune evasion and anti-PD-1 therapy resistance in renal cell carcinoma
Article Snippet: .. Protein A/G Magnetic Beads (MedChemExpress, USA) were then added and incubated for 2 h at 4 °C with rotation. ..

Article Title: LIPE‐AS1 Facilitates Prostate Cancer Progression via Suppressing Cuproptosis Through the miR‐330‐3p/MGAT5 Axis
Article Snippet: .. Following centrifugation, the clarified lysates were incubated overnight at 4°C with Protein A/G magnetic beads (MedChemExpress, Monmouth Junction, NJ, USA; Cat. No. HY‐P700331A) pre‐bound to an anti‐ FLAG antibody (Proteintech, Wuhan, China; Cat. No. 20543‐1‐AP; rabbit; 1:10000). ..

Article Title: TRIM28 Increases RFC4 Protein Expression to Promote Triple‐Negative Breast Cancer Progression
Article Snippet: .. The supernatants were incubated overnight at 4°C with the indicated primary antibodies and Protein A/G magnetic beads (MedChemExpress). ..

Article Title: PREX1 regulates renal fibrosis through the RPTOR-mTORC1 signaling pathway.
Article Snippet: For Co-IP assays, HK-2 cells were lysed in NP-40 buffer (Beyotime) containing protease and phosphatase inhibitors. .. Cell lysates containing 1 mg of total protein were incubated overnight at 4 ◦C with 5 μg of antiPREX1 antibody (Proteintech, Cat# 22460–1-AP) or control IgG (Proteintech, Cat# 30000–0-AP), followed by incubation with Protein A/G magnetic beads (MedChemExpress, Cat# HY-K0205) for 2 h at 4 ◦C. ..

Article Title: PREX1 regulates renal fibrosis through the RPTOR-mTORC1 signaling pathway.
Article Snippet: For Co-IP assays, HK-2 cells were lysed in NP-40 buffer (Beyotime) containing protease and phosphatase inhibitors. .. Cell lysates containing 1 mg of total protein were incubated overnight at 4 ◦C with 5 μg of antiPREX1 antibody (Proteintech, Cat# 22460–1-AP) or control IgG (Proteintech, Cat# 30000–0-AP), followed by incubation with Protein A/G magnetic beads (MedChemExpress, Cat# HY-K0205) for 2 h at 4 ◦C. ..

Article Title: Three-dimensional chromatin architecture is rapidly restored after DNA replication
Article Snippet: .. The supernatant was collected, 5% was stored as input material, and the rest was applied to Protein A/G magnetic beads (MedChemExpress) pre-bound to antibodies and allowed to incubate overnight at 4 °C with rocking. ..

Article Title: A core transcriptional regulatory circuitry controls super-enhancer-driven activation of LGR5 in colorectal cancer.
Article Snippet: .. Antibody-bound chromatin was captured by incubating with pre-cleared Protein A/G Magnetic Beads (MCE) for at least 4 h at 4 °C with agitation. ..

Article Title: In vivo CRISPR screens identify dual functions of DNMT3A in mediating tumor immune evasion.
Article Snippet: Briefly, cells were lysed in RIPA buffer (Beyotime, P0013C) supplemented with phenylmethylsulfonyl fluoride (PMSF; MCE, HY-B0496) and phosphatase inhibitors I and II (MCE, HY-K0021, and HY-K0022). .. For IP assays, cell lysates were incubated with protein A/G magnetic beads (MCE, HY-K0202), anti-Flag magnetic beads (MCE, HY-K0207) or anti-DNMT3A antibody (ABclonal, A19659) overnight at 4 °C with gentle rotation. ..

Article Title: Targeting NSUN5-mediated m5C-RNA modification overcomes sunitinib resistance in renal cell carcinoma by suppressing Fibronectin/PI3K/AKT signaling
Article Snippet: In brief, mRNA was extracted with the Hieff NGS mRNA Isolation Master Kit (#12603ES24; Yeasen, China) and fragmented with 10× Fragmentation Reagent (AM8740; Ambion, USA) at 70 °C for 7 min. .. Following this, 5 μg of the anti-m5C antibody (#ab73938; Abcam) was combined with 50 μL of protein A/G magnetic beads (#HY-K0202; MCE) at RT for 30 min, followed by incubation with fragmented RNA at 4 °C overnight. ..

Article Title: KDM4B-mediated AP2A1-K217 demethylation promotes immune evasion and anti-PD-1 therapy resistance in renal cell carcinoma
Article Snippet: .. Protein A/G Magnetic Beads (MedChemExpress, USA) were then added and incubated for 2 h at 4 °C with rotation. ..

Article Title: LIPE‐AS1 Facilitates Prostate Cancer Progression via Suppressing Cuproptosis Through the miR‐330‐3p/MGAT5 Axis
Article Snippet: .. Following centrifugation, the clarified lysates were incubated overnight at 4°C with Protein A/G magnetic beads (MedChemExpress, Monmouth Junction, NJ, USA; Cat. No. HY‐P700331A) pre‐bound to an anti‐ FLAG antibody (Proteintech, Wuhan, China; Cat. No. 20543‐1‐AP; rabbit; 1:10000). ..

Article Title: TRIM28 Increases RFC4 Protein Expression to Promote Triple‐Negative Breast Cancer Progression
Article Snippet: .. The supernatants were incubated overnight at 4°C with the indicated primary antibodies and Protein A/G magnetic beads (MedChemExpress). ..

Article Title: In vivo CRISPR screens identify dual functions of DNMT3A in mediating tumor immune evasion.
Article Snippet: Briefly, cells were lysed in RIPA buffer (Beyotime, P0013C) supplemented with phenylmethylsulfonyl fluoride (PMSF; MCE, HY-B0496) and phosphatase inhibitors I and II (MCE, HY-K0021, and HY-K0022). .. For IP assays, cell lysates were incubated with protein A/G magnetic beads (MCE, HY-K0202), anti-Flag magnetic beads (MCE, HY-K0207) or anti-DNMT3A antibody (ABclonal, A19659) overnight at 4 °C with gentle rotation. ..

Article Title: LIPE‐AS1 Facilitates Prostate Cancer Progression via Suppressing Cuproptosis Through the miR‐330‐3p/MGAT5 Axis
Article Snippet: .. Following centrifugation, the clarified lysates were incubated overnight at 4°C with Protein A/G magnetic beads (MedChemExpress, Monmouth Junction, NJ, USA; Cat. No. HY‐P700331A) pre‐bound to an anti‐ FLAG antibody (Proteintech, Wuhan, China; Cat. No. 20543‐1‐AP; rabbit; 1:10000). ..